One clone was unique and the other was ampicillin susceptible. Acinetobacter baumannii update linkedin slideshare. Carbapenemnonsusceptible acinetobacter baumannii of. Shortread wholegenome sequencing was conducted to determine the genetic basis. Acinetobacter baumannii is a troublesome opportunistic pathogen with a high capacity for clonal dissemination. Multidrugresistant acinetobacter baumannii harboring oxa. In recent years, bacteriophages and their derivatives, such. Phenotypic and molecular characterisations of carbapenem. The platform of acinetobacter baumannii mlst databases provides an open access to all the data.
The enormous increase in multidrug resistance among hospital isolates and the recent emergence of pandrugresistant strains underscores the urgency to understand how a. Investigation of a possible outbreak of carbapenem. Apr, 2016 clonal dissemination is characteristic of the important nosocomial pathogen acinetobacter baumannii, as revealed by previous multilocus sequence typing mlst studies. To assess the possibility of using the mlst scheme for acinetobacter genomic species tu, separate analyses were performed for a. Complete genome sequence of multidrugresistant acinetobacter baumannii strain 16562, which forms sturdy biofilm. It can be an opportunistic pathogen in humans, affecting people with compromised immune systems and is becoming increasingly important as a hospital derived infection nosocomial. To further expand the limited multilocus sequence typing mlst database for acinetobacter baumannii, 53 clinical isolates from various outbreaks in europe and the usa, collected between 1991 and 2004, plus the a. While we were isolating bartonella quintana from body lice, 40 acinetobacter baumannii strains were also isolated and genotyped.
Mlst analysis in easy database setup an mlst database can be easily set up based on any publicly available mlst scheme definition for e. Acinetobacter baumannii is a notorious opportunistic pathogen mainly. Introduction acinetobacter baumannii is a gramnegative, nonfermentative, oxidasenegative coccobacillus. Studies on the clonal relatedness of isolates could lay the foundation for effective infection control. Full text acinetobacter baumannii efflux pumps and. The platform of acinetobacter baumannii mlst databases provides an open access to all the data and allows submissions of novel sequences. This is commonly referred to as the oxford scheme because it was hosted on the pubmlst site at the. Escherichia coli, salmonella enterica, klebsiella pneumoniae, campylobacter, acinetobacter baumannii, pseudomonas aeruginosa or any other organism available from an online repository, simply by. Molecular epidemiology of carbapenemresistant acinetobacter. Jul 07, 2017 acinetobacter baumannii becoming a superbug increasing carbapenem resistance among acinetobacter species is particularly troubling, because it is very often associated with multidrug resistance and because it is occurring in the context of increases in the incidence of acinetobacter infection 772017 dr. Complete genome analysis of three acinetobacter baumannii clinical isolates in china for insight into the diversification of drug resistance elements. Antibiotic resistant strains of acinetobacter baumannii have been responsible for an increasing number of nosocomial infections including bacteremia and ventilatorassociated pneumonia. Icaacicc 2015 september 17 21, 2015 san diego, ca san. Characterization of epidemiologically unrelated acinetobacter.
Bacterial identification and antibiotic sensitivity tests were performed. Acinetobacter baumannii is a globally distributed nosocomial pathogen that has gained interest due to its resistance to most currently used antimicrobials. Pdf genome sequence of acinetobacter baumannii tyth1. The genus acinetobacter consists of 22 nomenspecies and at least 11 additional putative species. Backgroundacinetobacter baumannii is a notorious opportunistic pathogen mainly associated with hospitalacquired infections. Multidrugresistant acinetobacter baumannii harboring oxa24 carbapenemase, spain joshi acosta, 1 maria merino, esther viedma, margarita poza, francisca sanz, joaquin r. Oct 14, 2015 acinetobacter baumannii is a globally distributed nosocomial pathogen that has gained interest due to its resistance to most currently used antimicrobials. In february 2006, a patient colonized with a multidrugresistant sequence type 56 acinetobacter baumannii strain was admitted to a hospital in madrid, spain. This server controls the allelic nomenclature of core genome mlst bacterial gene schemes. The ability of this organism to gain multiple virulence factors. Otero, fernando chaves, and german bou in february 2006, a patient colonized with a multidrugresistant sequence type 56 acinetobacter baumannii strain. The incorporation of the efp primer pair expanded the discrimination power of the pcresims acinetobacter baumannii mlst assay to include the identification and typing of other acinetobacter species.
Acinetobacter baumannii becoming a superbug increasing carbapenem resistance among acinetobacter species is particularly troubling, because it is very often associated with multidrug resistance and because it is occurring in the context of increases in the incidence of acinetobacter infection 772017 dr. It may also cause urinary tract and wound infections. It can be an opportunistic pathogen in humans, affecting people with compromised immune systems, and is becoming increasingly important as a hospitalderived infection. To better understand the genetic variation and antibiotic resistance mechanisms of this bacterium at the genomic level, we reported high. The complete genome and phenome of a communityacquired acinetobacter baumannii. Acinetobacter outbreaks involving multidrugresistant strains have occurred worldwide 5, 6, 14, 28, 36, 39, 46, 47. Investigation of a possible outbreak of carbapenemresistant. Comparative analysis of the two acinetobacter baumannii. Phylogenetic analysis revealed that ihit7853 belonged to the globally distributed international clone ic1 and mlst type st1st231 pasteuroxford mlst scheme. Acinetobacter is a genus of gramnegative bacteria belonging to the wider class of gammaproteobacteria. Jul, 20 acinetobacter baumannii acinetobacter baumannii is a gram negative bacteria. Multilocus sequence typing analysis of carbapenemresistant acinetobacter baumannii in a chinese burns institute acinetobacter baumannii is a leading pathogen responsible for nosocomial infections.
Multidrugresistant acinetobacter baumannii harboring oxa24. This tool will allow the molecular surveillance of nonclassic pathogens other than a. This strain spread rapidly and caused a large outbreak in the hospital. Acinetobacter baumannii is a nosocomial pathogen increasingly affecting the critically ill patients and represents a major public health challenge. Interestingly, the sequence type of the first isolated 5. The acinetobacter baumannii global clone 1 isolate ab3070294, recovered in the usa in 1994, and the global clone 2 gc2 isolate acicu, isolated in 2005 in italy, were among the first a. Database for the ampc alleles in acinetobacter baumannii. Multilocus sequence typing mlst databases and software.
Acinetobacter baumannii acinetobacter baumannii is a gram negative bacteria. Acinetobacter species assigned to the acinetobacter calcoaceticusbaumannii acb complex, are gramnegative bacteria responsible for a large number of human infections. Relatedness determinations were carried out as previously described. We provide means of accessing these databases in common formats suitable for easy linking and importation to local resources. Acinetobacter baumannii mlst pasteur database pubmlst.
Acinetobacter species assigned to the acinetobacter calcoaceticus baumannii acb complex, are gramnegative bacteria responsible for a large number of human infections. The name of the folder is the scheme name, say saureus for staphylococcus aureus. This study aimed to analyze the clinical characteristics of patients and molecular mechanisms of the first outbreak mainly caused by sequence types sts 208 multidrug resistant mdr acinetobacter baumannii in china. Comparative genomic analysis of acinetobacter baumannii.
This germ may be found on skin or in food, water, or soil. A total of 10 clinical samples were collected from 5 patients who were involved in the outbreak. St208, st218, st231, st348, and two new sequence types suggesting that more than one clone is circulating palestinian hospitals. Meningitis research foundation mrf has commissioned the creation of a comprehensive online library of the genomes of all meningococcal disease isolates for 201011 in england, wales and northern ireland.
A new set of primers for rpob gene amplification is require to include a. Acinetobacter baumannii is an important nosocomial pathogen that poses a serious health threat to immunecompromised patients. Species of the acinetobacter calcoaceticusacinetobacter baumannii complex are important human pathogens which can be recovered from animals and food, potential sources for their dissemination. Pcr and sequencing confirmed that bla oxa23 was the only acquired carbapenemase gene in all isolates. It is urgently necessary to search for alternatives to conventional antibiotics for control of severe a. The aim of the present study was to characterise the acinetobacter isolates recovered from market meat samples in peru. Bacteremic pneumonia bp caused by this organism has a higher mortality compared to other organisms. Acinetobacter baumannii is an emerging human pathogen and a significant cause of nosocomial infections among hospital patients worldwide. Acinetobacter baumannii can cause serious infections in the lungs, blood, and brain. Acinetobacter baumannii is an important human pathogen usually associated with severe hospitalacquired infections. It is typically a short, almost round, rod shape coccobacillus. The sequence definition database contains allele sequence and mlst profile definitions whereas the isolate database contains provenance and epidemiological information.
These databases host mlst schemes and isolate data, increasingly including whole genome sequences. Development of a multilocus sequence typing scheme for characterization of clinical isolates of acinetobacter baumannii. Acinetobacter baumannii is a bacterial species that can survive on moist and dry surfaces. This study used a diverse collection of epidemiologically unrelated acinetobacter baumannii isolates to compare the robustness of a multilocus sequence typing mlst scheme, based on conserved regions of seven housekeeping genes, glta, gdhb, reca, cpn60, rpod,gyrb, and gpi, with that of sequencebased typing of bla oxa51like genes sbtbla. Multilocus sequence typing mlst analysis applied maths. Emergence of carbapenemresistant acinetobacter baumannii. This study used a diverse collection of epidemiologically unrelated acinetobacter baumannii isolates to compare the robustness of a multilocus sequence typing mlst scheme, based on conserved regions of seven housekeeping genes, glta, gdhb, reca, cpn60, rpod, g yrb, and gpi, with that of sequencebased typing of bla oxa51like genes sbt bla oxa51like. Molecular epidemiology of clinical acinetobacter baumannii.
Comprises fortyeight distinctive alleles of the ampc locus. Due to its rapid ability to develop multidrug resistance mdr, a. Inaccurate multilocus sequence typing of acinetobacter baumannii. The isolates covered the time period of the study between 2006 and 2014 such that one isolate was from the year 2006, two were from the year 2007, one was from the. Here, we announce the draft genome sequences of two livestockassociated isolates recovered from sewage water from a poultry slaughterhouse in germany. Identification of acinetobacter species and genotyping of. This site hosts publicly accessible multilocus sequence typing mlst databases and software. The centers for disease control and prevention cdc recognizes multidrug resistant mdr a. To compare the two acinetobacter baumannii multilocus sequence typing mlst schemes and to assess their suitability to aid in outbreak analysis we investigated the molecular epidemiology of 99. Phylogenetic and genomic diversity in isolates from the. In this study a multilocus sequence typing mlst scheme for acinetobacter baumannii was developed and evaluated by using 40 clinical a.
Acinetobacter baumannii efflux pumps and antibiotic resistance seyyed naser abdi,1,2 reza ghotaslou,3 khudaverdi ganbarov,4 ahmad mobed,1 asghar tanomand,5 mehdi yousefi,3 mohammad asgharzadeh,6 hossein samadi kafil1 1drug applied research center, faculty of medicine, tabriz university of medical sciences, tabriz, iran. Development of a multilocus sequence typing scheme for. The database is openly accessible at the pubmlst platform. This site uses two linked databases powered by the bigsdb genomics platform. To better understand the genetic variation and antibiotic resistance mechanisms of. The population structure of acb has been studied using two 7gene mlst schemes, introduced by bartual and coworkers oxford scheme and by diancourt and coworkers pasteur scheme. Carbapenem resistance in acinetobacter baumannii is most often encoded by oxacillinases oxas and less frequently by metallo. Genomewide recombination drives diversification of. The first outbreak caused by acinetobacter baumannii st208. Two isolates did not have an exact st match in the database.
Draft genome sequences of acinetobacter baumannii isolates. Each mlst scheme exists in a folder withing the mlstdbpubmlst folder. Genomic analysis of antibiotic resistance for acinetobacter. To compare the two acinetobacter baumannii multilocus sequence typing mlst schemes and to assess their suitability to aid in outbreak analysis we.
Headtohead comparison of two multilocus sequence typing. Jan 30, 2020 each mlst scheme exists in a folder withing the mlst dbpubmlst folder. Identification and characterization of capsule depolymerase. In hospitalized patients, acinetobacter baumannii frequently colonizes. Acinetobacter baumannii is a typically short, almost round, rodshaped coccobacillus gramnegative bacterium.
Genome sequence of oxa23 producing acinetobacter baumannii. This is commonly referred to as the oxford scheme because it was hosted on the pubmlst site at the university of oxford. Aug 16, 2011 acinetobacter baumannii is an emerging human pathogen and a significant cause of nosocomial infections among hospital patients worldwide. Characterization of carbapenemresistant acinetobacter. Shortread wholegenome sequencing was conducted to determine the genetic basis of their antimicrobial resistance phenotype. Acinetobacter baumannii infection discharge care what. Acinetobacter species are oxidasenegative, exhibit twitching motility, and occur in pairs under magnification they are important soil organisms, where they contribute to the mineralization of, for example, aromatic compounds. Clinicians should be alert for this strain because its spread would have serious health consequences.
To compare the two acinetobacter baumannii multilocus sequence typing mlst schemes and to assess their suitability to aid in outbreak analysis we investigated the molecular epidemiology of 99 acinetobacter baumannii isolates representing outbreakrelated and sporadic isolates from 24 hospitals in four different countries germany, poland, sweden, and turkey. Clonal diversity of acinetobacter baumannii clinical isolates. We announce the establishment of a database for the ampc locus in a. The hosting of both schemes within a single bigsdb database, which can.
Inaccurate multilocus sequence typing of acinetobacter. Genomewide recombination drives diversification of epidemic. Acinetobacter baumannii an update linkedin slideshare. Background the emergence of multidrug or extensively drugresistant acinetobacter baumannii has made it difficult to treat and control infections caused by this bacterium. Development of a multilocus sequence typing scheme for characterization of. An acinetobacter baumannii infection is caused by the acinetobacter baumannii bacteria germ. Pathogenic acinetobacter species including the novel.
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